Transfection Reagents
New England Biolabs, Inc. NEB® 10-beta Competent E. coli (High Efficiency) – 6 x 0.2 ml/tube
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations.
- High efficiency strain ideal for cloning large plasmids and BACs
- Also available in chemically-competent format (single-use and 200 µl vials)
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Invivogen dsDNA Naked, Complexed with Transfection Reagent, 100 µg
Poly(dA:dT) is a poly(deoxyadenylic-deoxythymidylic) acid sodium salt. It is a repetitive synthetic double-stranded DNA sequence of poly(dA-dT); poly(dT-dA) and a synthetic analog of B-DNA. Poly(dA:dT) is recognized by several sensors, including DAI, LRRFIP1 and AIM2. Poly(dA:dT) is also indirectly detected by RIG-I. The activity of poly(dA:dT) is tested using the reporter cell line B16-Blue IFN/. Solubility: 2 mg/ml in physiological water. Unit Size:100 g. 86828-69-5
Expression Systems Transfection Medium, 100ml
Expression Systems Transfection Medium is designed to complement ESF 921 and ESF AF insect cell culture media for the cotransfection or transfection step in baculovirus vector production. Transfection Medium is a serum-free and animal-free formulation that facilitates vector DNA uptake by the insect host cells. Realize higher titers by using a formulation designed to work with ESF 921 and ESF AF.
• Serum-free, animal-free formulation works seamlessly with ESF 921 and ESF AF
• Appropriate for PEI or lipofection mediated transfection
• Optimized for use with Expres2 TR Transfection Reagent
• Increases virus vector production and transient expression
• Available in 20 mL or 100 mL
• In stock for immediate shipment
• To download Instructions for Use and/or COA please visit: www.expressionsystems.com
Selleck Chemical LLC Ro-3306 10mg 872573-93-8
RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases. RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis. *For Research & Development use only. Product is not intended for drug, household, or human consumption.
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 3 TRANSFECTION REAG
NC1250221 DHARMAFECT 3 TRANSFECTION REAG
Lonza Walkersville SE Cell Line 96-well Nucleofector Kit (960 RCT)
The SE Cell Line 96-well Nucleofector Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL SE Cell Line Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)10 x 96-well Nucleocuvette Plate (20 μL)
HORIZON DISCOVERY BIOSCIENCES LIMITED Accell Green Non-targeting Control siRNA, 20nmol
Accell Green Non-targeting Control siRNA provides qualitative assessment of Accell siRNA uptake as determined by fluorescent microscopy to evaluate cytoplamic localization of the dye-labeled Accell siRNA or FACS analysis to determine general uptake. Non-targeting siRNA reagents are designed to aid in the interpretation of experiments with Accell siRNA reagents as highly reliable controls for assessment of baseline cellular responses.
Boca Scientific Inc LentiBlast Premium Transduction Enhancer, 1500ul
LentiBlast Premium Transduction Enhancer is designed to increase lentiviral infection and transduction efficiency in a wide range of cell types, from CD34+ hematopoietic stem cells to primary cells or cell lines such as HT1080 or SH SY5Y. LentiBlast Premium is composed of only one reagent, allowing for a new single-step protocol. With LentiBlast Premium, neutralizing electrostatic repulsions between membranes and viral particles and enhancing viral fusion with cell membranes can occur simultaneously. Due to a favorable membrane permeable effect limiting the transmembrane potential changes, LentiBlast Premium is non-toxic and totally compatible with cell viability.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3958052 CACO-2 CELL LINE HUMAN
Lonza Walkersville Human Stem Cell Nucleofector Kit 1
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 1 is one of our two kits suited for efficient transfection of human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 1, 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes