Transfection Reagents
SignaGen Laboratories GENJET DNA REAGENT K562 1ML
GenJet DNA In Vitro Tranfection Reagent for K562 was pre-optimized for most efficiently transfecting K562 cells.
Andwin Scientific FectoPRO DNA Transfection Kit
The FectoPRO Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount.
Sino Biological Sinofection Transfection Reagent 1 mL
Sinofection is a superior cationic polymer-based transfection reagent. It has been successfully applied to transfection at various scales over a broad range of cell lines. Compared with the leading transfection reagents on the market, Sinofection yields higher protein expression level and exhibits lower cytotoxicity. It is an ideal choice for recombinant protein production for both transient and stable transfection. Its extensive compatibility with serum, cell type, scale, and storage temperature offers you ease and assurance for research and production.
SignaGen Laboratories GENJET DNA REAGENT B16 1ML
GenJet DNA In Vitro Tranfection Reagent for B16 was pre-optimized for most efficiently transfecting B16 cells.
STEMCELL Technologies Resazurin (Sodium Salt), 5 g
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media and further differentiated into cells of all three germ layers (Figure 7).
LGC GENOMICS LLC (Lucigen) TransforMax EC100D pir-116 Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μl)
New England Biolabs, Inc. dam-/dcm- Competent E. coli – 6 x 0.2 ml/tube
Methyltransferase deficient chemically competent E. coli cells suitable for growth of plasmids free of Dam and Dcm methylation. Note that dam- strains are not recommended as a host for primary cloning/ligation. The dam mutation can result in an increased mutation rate in the cell and a reduction in the transformation efficiency. DNA should be maintained in a dam+ strain unless there is a specific need for DNA free of Dam or Dcm methylation.
Lonza Walkersville Cell Line Optimization 4D-Nucleofector X Kit
The Cell Line Optimization 4D-Nucleofector X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X Unit.Benefits:Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experimentOptimal Nucleofection conditions determined in the 16-well Nucleocuvette Strips are transferable to the 100 μL single Nucleocuvette in the 4D-Nucleofector X UnitConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X UnitContent:0.675 mL Nucleofector Solution SE0.675 mL Nucleofector Solution SF0.675 mL Nucleofector Solution SG3 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0) 4 x 20 µL 16-well Nucleocuvette Strips
SignaGen Laboratories CALFECTIN DNA REAGENT 1ML
CalFectin Mammalian DNA Tranfection Reagent is a pre-optimized and refined version of traditional calcium phosphate transfection reagent. With a new chemistry, CalFectin Reagent contains much lower concentration of calcium, leading to much lower cytotoxicity with boosted transfection efficiency.
Kyfora Bio Amplifect 5 mL RUO
Introducing AmpliFect our new transfection enhancer that is designed to effortlessly integrate with all our PEI transfection reagents AmpliFect boosts viral vector (AAV LV) recombinant protein and monoclonal antibody (mAb) titers by more than 3x delivering significant improvements in yield This titer increase not only elevates your productivity but also helps lower manufacturing costs When used in combination with our affordable PEI transfection reagents AmpliFect offers superior performance and efficacy compared to other transfection solutions on the market
Dojindo Molecular Technologies Inc HilyMax (1ML x 1) is a high transfection efficiency reagent, and is the best reagent for cell signaling research.
HilyMax is a gene transfection reagent that forms a liposome to be used for highly efficient gene transfection to a wide variety of cells. HilyMax readily interacts with DNA because cationic liposomes (+) and anionic DNA (-) spontaneously form DNA liposome complexes. The overall charge of DNA-HilyMax complex is positive, such that the DNA-HilyMax complex is electrostatically bound on an anionic cell surface and introduces DNA into the cell by endocytosis. Since the serum in the growth medium does not interfere with the transfection using HilyMax, no exchange of the medium during the transfection is required. HilyMax does not contain biological components that might interfere with the transfection.
LAMDA BIOTECH INC Tranfection reagent: LipoFexin
LipoFexin is a lipid-based transfection reagent that forms complex with DNA or RNA, and transports the complex into cells. It works on a variety of adherent and suspension cell lines. It can be used for transfection of either DNA or RNA into eukaryotic cells in culture media with or without serum. LipoFexin has been compared to work at the similar way and efficiency as the Lipofectamine 2000 transfection reagent. LipoFexin has the following Special Features: • Superior transfection efficiency for a broad range of cell lines. • No requirement of removal of serum from culture medium. • No requirement for washing or changing of medium after transfection. • Low cytotoxicity.
ABclonal Technology DLDH/DLD Rabbit pAb
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)