Transfection Reagents
Andwin Scientific FectoPRO DNA Transfection Kit
The FectoPRO Transfection Kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount.
Lonza Walkersville P3 Primary Cell 4D-Nucleofector X Kit L
The P3 Primary Cell 4D-Nucleofector X Kit L is one of our five 4D-Nucleofector Kits suited for transfecting primary cells in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.Benefits:High transfection efficiencies in for primary cellsCells maintain functionality post transfectionConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow throughput applicationsContent:2 x 0.675 mL P3 Primary Cell Nucleofector Solution2 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)12 x 100 µL Nucleocuvette Vessels12 x Single use pipettes
Supply Solutions Roche - X-tremeGENE 9 DNA Transf. Reag. 0.4 ml; Polymer reagent for transfecting common cell lines; pkg 0.4 mL (06365779001) pkg of 1.0 mL (06365787001) pkg of 5 x 1.0 mL (06365809001)
Roche - X-tremeGENE 9 DNA Transf. Reag. 0.4 ml; Polymer reagent for transfecting common cell lines; pkg 0.4 mL (06365779001) pkg of 1.0 mL (06365787001) pkg of 5 x 1.0 mL (06365809001); Hazardous: Y
System Biosciences LLC TRANSFECTION-READY CAS9 MRNA
Transfection-ready Cas9 SmartNuclease mRNA (Eukaryotic version) 20 ug
Agilent Technologies SURE COMPETENT CELLS
SURE Competent Cells. For routine cloning of DNA with secondary structures, > /= 5 x 108 transformants/microgram. Restriction-Minus Competent Cells for Most Eukaryotic DNA. F- derivative for BAC cloning.
Agilent Technologies GENEJAMMER TRANSFECT REAGENT
GeneJammer Transfection Reagent is a proprietary formulation of polyamine and other components in 80% ethanol ideal for transient or stable transfections in a wide variety of cell lines .Fast, easy to use with low cytotoxicity
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
New England Biolabs, Inc. pUC19 Vector – 50 µg
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases.
Lonza Walkersville Human Stem Cell Nucleofector Kit 1
The Nucleofector 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell Nucleofector Kit 1 is one of our two kits suited for efficient transfection of human stem cells. Due to significant diversity between different human stem cell clones, we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific clone.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Stem Cells0.5 mL Supplement 1, 30 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Takara Bio XFECT TRANSFECTION REAGENT
Xfect Transfection reagent creates bio-degradable nanoparticles for superior transfection efficiency of plasmid DNA into mammalian cells.
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cells High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 96-well Nucleocuvette Plate (20 μl)